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KMID : 0378019780210010053
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New Medical Journal
1978 Volume.21 No. 1 p.53 ~ p.72
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An Experimental Study for the Effect of ACTH on the Adrenal Cortex,Bone Marrow and Lymphoid Tissue with Rabbit
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Abstract
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Many studies for the effect of ACTH were executed with various animals. The histological changes of the adrenal cortex were illustrated in relation to the endocrinological and biochemical action. Reports for the effect on the peripheral blood were also established but the mechanism was in controversy. Author attempted to clarify the histological changes of the adrenal cortex and the mechanism of the effect on the individual differential cells of leukocytes, also tried to pursue the changes of bone marrow, lymphnode and spleen under the influence of ACTH or with surgical stress. Thirty eight healthy young adult male rabbits weighing from 1.80 kg to 2.20 kg were utilized. Animals were sacrificed by the injection of air into the vein of the ear, and then the adrenal gland, femoral bone marrow, spleen and mesenteric lymphnodes were examined. The findings and results obtained on this experiment were as follows; 1) The microscopic structure of the adrenal cortex following stimulation by ACTH or with surgery was revealed; the cells of zona fasciculata were initially clear and pale staining predominently became compact and dark staining and then the change was extended to the zona glomerulosa, which was related to the amount and duration of administered ACTH, especially predominant in addition to surgery. In the group of rabbits administered large amount of ACTH, although compact cells were proliferated, tiny lipid like material was increased and vacuolated cells were appeared which was presumably resulted from the degeneration of cytoplasm. 2) The bone marrow showed marked decrease in cellularity, erythroid series, megakaryocytes and eosinophils, but fatty marrow and myeloid series were remarkably increased. Lymphocytes, were slightly increased. The above mentioned changes were more remarkable in the fifth group of animal. 3) Lymphoid tissue was markedly reduced, and observed follicular atrophy, sinusoidal edema, congestion and decrease in number of lymphocytes. In areas some lymphocytes showed karyorrhexis or karyolysis. Such findings were predominant in the fifth group of animal. 4) Spleen was moderately atrophied and its lymphoid tissue disclosed a considerable decrease in amount. There was congestion, edema, partial proliferation of fibrous tissue, precipitation of blood pigments and infiltration of polymorphonuclear-leukocytes and macrophages in the parenchyme. These findings were more pronounced in the fifth group of animal. 5) In peripheral blood with the injection of ACTH and or laparotomy produced absolute lymphopenia and eosinophenia, however circulating neutrophils were markedly increased in number.
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